Generator

Part:BBa_J23005:Design

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-06-23)

[FRT-CmR-FRT]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 35
    Illegal XbaI site found at 965
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 35
    Illegal XbaI site found at 965
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 35
    Illegal XbaI site found at 965
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None

Source

PCR ca1005F/R on pKD3 (1066 bp, EcoRI/PstI)

sub into pSB1A2 (EcoRI/PstI)

product is pSB1A2-Bca1005

ca1005F Forward Biobrick for pKD3 FRT-Cm-FRT

gacttgaattcgcggccgcttctagagtgtaggctggagctgcttc

ca1005R Reverse Biobrick for pKD3 FRT-Cm-FRT

cctaactgcagcggccgctactagtacatatgaatatcctccttag

References